Article
Development of a new HLA-DRB real-time PCR typing method.
Human immunology - 1 Jan 2005
Casamitjana Natàlia, Faner Rosa, Santamaria Albert, Colobran Roger, Ribera Anna, Pujol-Borrell Ricardo, Juan Manel, Palou Eduard
Abstract excerpt
Polymerase chain reaction (PCR)-based human leukocyte antigen (HLA) typing methods currently used in most histocompatibility laboratories, such as PCR-sequence-specific primers (PCR-SSP) and PCR-sequence-specific oligonucleotide probes (PCR-SSO), are time-consuming and are at risk of contamination during the post-PCR process. The aim of this study was to develop a real-time PCR (rtPCR)-based HLA-DRB1 and...
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