High resolution HLA-DRB1 SSP typing for cadaveric donor transplantation.

doi:10.1111/j.1399-0039.1995.tb02413.x

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Article

High resolution HLA-DRB1 SSP typing for cadaveric donor transplantation.

Tissue antigens - 1 Jan 1995

Savelkoul P H, de Bruyn-Geraets D P, van den Berg-Loonen E M

Abstract excerpt

An HLA-DRB1 typing procedure by means of sequence-specific primer (SSP) amplification was developed for 65 different DRB1 subtypes. Subtyping is achieved by the performance of two subsequent PCR assays (PCR-1 assay and PCR-2 assay) using a limited number of reactions. The PCR-1 assay determined l...

Topics

Alleles
Base Sequence
Cadaver
Cell Line
DNA Primers
HLA-DR Antigens
HLA-DRB1 Chains
Histocompatibility Testing
Humans
Molecular Sequence Data
Organ Transplantation
Polymerase Chain Reaction
Tissue Donors

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