Article
High resolution HLA-DRB1 SSP typing for cadaveric donor transplantation.
Tissue antigens - 1 Jan 1995
Savelkoul P H, de Bruyn-Geraets D P, van den Berg-Loonen E M
Abstract excerpt
An HLA-DRB1 typing procedure by means of sequence-specific primer (SSP) amplification was developed for 65 different DRB1 subtypes. Subtyping is achieved by the performance of two subsequent PCR assays (PCR-1 assay and PCR-2 assay) using a limited number of reactions. The PCR-1 assay determined low-resolution HLA-DRB1 typing, i.e. the serologically defined specificities DR1, 2, 3, 4, 11, 12, 6, 7, 8, 9 and 10....
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