Article
HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours.
Tissue antigens - 1 Mar 1993
Olerup O, Aldener A, Fogdell A
Abstract excerpt
In the present study PCR primers were designed for detecting all phenotypically expressed DQB1 and DQA1 allelic variability, 19 and 10 alleles, respectively, by PCR amplification with sequence-specific primers (PCR-SSP). For DQB1 typing, each sample was amplified by a first set of 14 PCR primer p...
Topics
- Adult
- Alleles
- Base Sequence
- Cell Line
- Child
- Consensus Sequence
- DNA Probes, HLA
- Genotype
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- HLA-DQ beta-Chains
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- Reproducibility of Results
- Sensitivity and Specificity