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HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours.

Tissue antigens - 1 Mar 1993

Olerup O, Aldener A, Fogdell A

Abstract excerpt

In the present study PCR primers were designed for detecting all phenotypically expressed DQB1 and DQA1 allelic variability, 19 and 10 alleles, respectively, by PCR amplification with sequence-specific primers (PCR-SSP). For DQB1 typing, each sample was amplified by a first set of 14 PCR primer pairs, followed in some cases by two to six additional PCR reactions. The first 14 primer pairs allowed the...

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PMID: 8316943
DOI: 10.1111/j.1399-0039.1993.tb01991.x

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