HLA-AB typing by polymerase-chain reaction with sequence-specific primers: more accurate, less errors, and increased resolution compared to serological typing.

Abstract excerpt

Until recently, serological typing has been the primary technique used for HLA class I analysis. But because of limitations, molecular-typing techniques have replaced or supplemented the microlymphocytotoxicity test. It has been assumed that HLA class I serological typing was more accurate than serological HLA-DR typing; the latter has been shown to have 10-25% errors. But several studies have shown that HLA-AB...

Topics

• Alleles
• Bone Marrow Transplantation
• DNA Primers
• HLA Antigens
• HLA-A Antigens
• HLA-B Antigens
• HLA-C Antigens
• Hematologic Diseases
• Histocompatibility Testing
• Humans
• Kidney Transplantation