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Article

Rapid DNA typing for class II HLA antigens: subtyping of DRw52-associated DRB1 alleles.

Tissue antigens - 1 May 1993

Horne C, Keown P A

Abstract excerpt

Serologic typing for MHC class II antigens is incapable of identifying important subtypes for certain DRB1 alleles and occasionally leads to errors of assignment, particularly with the DR antigens associated with DRw52. To simplify DNA typing of DRw52-associated DRB1 alleles, we have developed a new rapid method using PCR-RFLP. The PCR-RFLP method is based on allele-specific amplification followed by digestion of...

Topics

Alleles
Base Sequence
DNA Primers
Genes, MHC Class II
HLA-D Antigens
HLA-DR Antigens
HLA-DR Serological Subtypes
HLA-DRB1 Chains
Histocompatibility Antigens Class II
Histocompatibility Testing
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 7901922
DOI: 10.1111/j.1399-0039.1993.tb02013.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7901922-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Rapid DNA typing for class II HLA antigens: subtyping of DRw52-associated DRB1 alleles.PMID 7901922Read ↗

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