Article
Comprehensive, serologically equivalent DNA typing for HLA-B by PCR using sequence-specific primers (PCR-SSP).
Tissue antigens - 1 Feb 1995
Bunce M, Fanning G C, Welsh K I
Abstract excerpt
Polymorphic products of HLA class I genes from the human major histocompatibility complex (MHC) are traditionally assigned by serology with additional heterogeneity detectable using one-dimensional isoelectric focusing (1D-IEF). With the increased availability of HLA class I DNA sequence information it has become feasible to genotype for class I by polymerase chain reaction utilising sequence-specific primers...
Topics
- Alleles
- Base Sequence
- Cell Line
- DNA Primers
- Genes, MHC Class I
- Genotype
- HLA-B Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction