Article
Rapid HLA-DQB typing by eight polymerase chain reaction amplifications with sequence-specific primers (PCR-SSP).
Human immunology - 1 Aug 1993
Bunce M, Taylor C J, Welsh K I
Abstract excerpt
Molecular genotyping of HLA class II genes using group-specific DNA amplification by the PCR followed by probing with (PCR-SSO) probes is too time consuming for the typing of cadaveric organ donors. Recently, amplification of DNA using PCR-SSP has proved a reliable and rapid method for typing HLA...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Primers
- Electrophoresis, Agar Gel
- False Positive Reactions
- Genotype
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Histocompatibility Testing
- Humans
- Kidney Transplantation
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Tissue Donors