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Article

Rapid cloning of HLA-A,B cDNA by using the polymerase chain reaction: frequency and nature of errors produced in amplification.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Ennis P D, Zemmour J, Salter R D, Parham P

Abstract excerpt

A method for cloning full-length HLA-A,B cDNA (1.1 kilobases) by using the polymerase chain reaction (PCR) is described. Six HLA-A,B alleles (HLA-A2, -A25, -B7, -B37, -B51, and -B57) were cloned, and their structures were determined. Multiple PCR clones for each allele were sequenced to obtain both an accurate consensus sequence and an "authentic" clone having that sequence. Sequences from 50 clones encoding five...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Cell Line
Cell Transformation, Viral
Cloning, Molecular
Exons
Genes, MHC Class I
HLA-A Antigens
HLA-B Antigens
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Polymerase Chain Reaction

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 2320591
DOI: 10.1073/pnas.87.7.2833
PMCID: PMC53785

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2320591-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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