Article
Identification of the HLA-DRB1*04, -DRB1*07, and -DRB1*09 alleles by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours.
Human immunology - 1 May 1992
Zetterquist H, Olerup O
Abstract excerpt
The clinical applicability of genomic HLA class II typing techniques has increased after the introduction of PCR-based typing strategies. In typing by PCR amplification using sequence-specific primers (PCR-SSP), amplification of specific alleles or groups of alleles is achieved, provided that the...
Topics
- Alleles
- Base Sequence
- Exons
- Genes, MHC Class II
- HLA-DR Antigens
- HLA-DRB1 Chains
- Heterozygote
- Histocompatibility Antigens Class II
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sensitivity and Specificity
- Sequence Tagged Sites