HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation.

Abstract excerpt

In most PCR-based tissue typing techniques the PCR amplification is followed by a post-amplification specificity step. In typing by PCR amplification with sequence-specific primers (PCR-SSP), typing specificity is part of the amplification step, which makes the technique almost as fast as serological tissue typing. In the present study primers were designed for DR "low-resolution" typing by PCR-SSP, i.e....

Topics

• Alleles
• Base Sequence
• Cadaver
• Genes, MHC Class II
• HLA-DR Antigens
• Histocompatibility Testing
• Humans
• Molecular Sequence Data
• Oligonucleotide Probes
• Polymerase Chain Reaction