Article
Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation.
Nucleic acids research - 1 Jul 1997
Wright D A, Park S K, Wu D, Phillips G J, Rodermel S R, Voytas D F
Abstract excerpt
We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E.coli strain with a non-reverting allele of pyrF that is also suitable for cloning (recA-, hsdR-). We demonstrate the utility of this...
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