Article
Replicon rescue: a novel strategy to clone the genomic DNA flanking insertions of integrating shuttle vector DNA.
Nucleic acids research - 15 Oct 1996
McMahon T L, Wilczynska Z, Barth C, Fraser D J, Pontes L, Fisher P R
Abstract excerpt
A novel cloning strategy, replicon rescue, was developed for cloning genes disrupted by plasmid insertions. After ligation to a tetracycline resistance cassette, fragments containing a bacterial origin of replication from the insertion are recovered in Escherichia coli because they replicate auto...
Topics
- Animals
- Cloning, Molecular
- DNA Restriction Enzymes
- Dictyostelium
- Escherichia coli
- Genes, Bacterial
- Genes, Fungal
- Genetic Vectors
- Klebsiella pneumoniae
- Mutagenesis, Insertional
- Mutation
- Plasmids