Article
An improved system for selection of forward mutations in an Escherichia coli supF gene carried by plasmids.
Journal of radiation research - 1 Dec 1998
Obata F, Nunoshiba T, Hashimoto-Gotoh T, Yamamoto K
Abstract excerpt
An improved system to examine forward mutations that occurred in the supF gene of Escherichia coli carried on a multicopy plasmid is described. The system was validated by measuring spontaneous mutations of supF plasmids propagated in wild-type, recA- and mutM- mutY- E. coli strains, the mutation frequencies of which were 1.3 x 10(-7), 6.3 x 10(-7) and 1.5 x 10(-6), respectively. Sequence analysis of the supF...
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