Article
Construction of plasmid vectors that facilitate subcloning and recovery of yeast and Escherichia coli DNA fragments.
Gene - 1 Jan 2000
Naumovski L, Friedberg E C
Abstract excerpt
We have constructed a plasmid vector (pNF2) which is a derivative of the multicopy yeast cloning vehicle YEp24. This derivative contains a single BamHI site flanked immediately on each side by SalI sites. The latter site was selected because it appears to be infrequent in yeast nuclear DNA. Thus, DNA fragments produced by partial digestion with enzymes (such as Sau3A) that cut at frequent intervals and leave...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Bacterial
- DNA, Fungal
- Escherichia coli
- Phenotype
- Plasmids
- Saccharomyces cerevisiae