Article
Isolation of a DNA fragment that is expressed as an amber suppressor when present in high copy number in yeast.
Gene - 1 Jan 2000
Calderon I L, Contopoulou C R, Mortimer R K
Abstract excerpt
A plasmid carrying a DNA sequence conferring amber suppression has been isolated from yeast pool 35 DNA. The plasmid YEp13-SUP suppresses the amber mutations rad50-1 and trp1-289 as well as other known amber mutations. This sequence is located on the 2.5-kb insert and is expressed only when the plasmid bearing it is present in high copy number. The suppressor sequence was shown to integrate close to the MAL4 gene...
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