Isolation of a DNA fragment that is expressed as an amber suppressor when present in high copy number in yeast.

doi:10.1016/0378-1119(84)90167-7

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Article

Isolation of a DNA fragment that is expressed as an amber suppressor when present in high copy number in yeast.

Gene - 1 Jan 2000

Calderon I L, Contopoulou C R, Mortimer R K

Abstract excerpt

A plasmid carrying a DNA sequence conferring amber suppression has been isolated from yeast pool 35 DNA. The plasmid YEp13-SUP suppresses the amber mutations rad50-1 and trp1-289 as well as other known amber mutations. This sequence is located on the 2.5-kb insert and is expressed only when the p...

Topics

Base Sequence
DNA Restriction Enzymes
DNA, Fungal
Genes, Fungal
Genotype
Mutation
Plasmids
Saccharomyces cerevisiae
Suppression, Genetic

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