Article
Direct complementation of Chlamydomonas mutants with amplified YAC DNA.
Genomics - 15 Sept 1996
Vashishtha M, Segil G, Hall J L
Abstract excerpt
We previously described the construction and characterization of a Chlamydomonas genomic library in yeast artificial chromosomes (YACs). Here we describe the isolation and genetic mapping of YACs at the FLA10 locus on the uni chromosome as well as isolation of a YAC spanning the PF14 locus on chromosome VI. Genetic mapping of YAC end clones by RFLP analyses in interspecific crosses reveals that YACs with a...
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