Article
Structural insights into the evolution of an antibody combining site.
Science (New York, N.Y.) - 13 Jun 1997
Wedemayer G J, Patten P A, Wang L H, Schultz P G, Stevens R C
Abstract excerpt
The crystal structures of a germline antibody Fab fragment and its complex with hapten have been solved at 2.1 A resolution. These structures are compared with the corresponding crystal structures of the affinity-matured antibody, 48G7, which has a 30,000 times higher affinity for hapten as a res...
Topics
- Antibodies, Catalytic
- Antibody Affinity
- Antibody Diversity
- Antigen-Antibody Complex
- Antigen-Antibody Reactions
- Binding Sites
- Binding Sites, Antibody
- Crystallography, X-Ray
- Evolution, Molecular
- Haptens
- Hydrogen Bonding
- Immunoglobulin Fab Fragments
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Structure, Secondary