Article
Mimicking somatic hypermutation: affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain.
Journal of molecular biology - 19 Jul 1996
Low N M, Holliger P H, Winter G
Abstract excerpt
Human antibodies can now be isolated from antibody repertoires displayed on the surface of filamentous bacteriophage in a process that mimics the primary immune response. Here we have attempted to mimic the secondary response, the natural process of affinity maturation of antibodies occurring in germinal centres, by multiple cycles of random mutation and selection. Phage displaying a human antibody fragment...
Topics
- Amino Acid Sequence
- Bacteriophages
- Base Sequence
- DNA, Recombinant
- Escherichia coli
- Haptens
- Humans
- Immunoglobulin Fragments
- Molecular Sequence Data
- Mutation
- Oxazolone