Article
Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten.
Molecular immunology - 1 Mar 2007
Furukawa Koji, Shimizu Takeyuki, Murakami Akikazu, Kono Ryo, Nakagawa Masatoshi, Sagawa Takuma, Yamato Ichiro, Azuma Takachika
Abstract excerpt
In order to quantitate the contribution of amino acid replacements to an increase in affinity during affinity maturation, we measured thermodynamic parameters of the antigen-antibody interaction for a group of anti-(4-hydroxy-3-nitrophenyl) acetyl monoclonal antibodies whose differences in amino acid sequences had arisen only from somatic hypermutation. We prepared a common ancestor and hypothetical intermediate...
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