Article
Genetic characterization of a mammalian protein-protein interaction domain by using a yeast reverse two-hybrid system.
Proceedings of the National Academy of Sciences of the United States of America - 17 Sept 1996
Vidal M, Braun P, Chen E, Boeke J D, Harlow E
Abstract excerpt
Many biological processes rely upon protein-protein interactions. Hence, detailed analysis of these interactions is critical for their understanding. Due to the complexities involved, genetic approaches are often needed. In yeast and phage, genetic characterizations of protein complexes are possi...
Topics
- Alleles
- Animals
- Binding Sites
- Carrier Proteins
- Cell Cycle Proteins
- DNA Primers
- DNA-Binding Proteins
- E2F Transcription Factors
- E2F1 Transcription Factor
- Fungal Proteins
- Gene Library
- Genes, Reporter
- Mammals
- Molecular Sequence Data
- Mutagenesis
- Polymerase Chain Reaction
- Protein Biosynthesis
- Protein Multimerization