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Deep Mutational Scanning of Protein-Protein Interactions Between Partners Expressed from Their Endogenous Loci In Vivo.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022

Dubé Alexandre K, Dandage Rohan, Dibyachintan Soham, Dionne Ugo, Després Philippe C, Landry Christian R

Abstract excerpt

Deep mutational scanning (DMS) generates mutants of a protein of interest in a comprehensive manner. CRISPR-Cas9 technology enables large-scale genome editing with high efficiency. Using both DMS and CRISPR-Cas9 therefore allows us to investigate the effects of thousands of mutations inserted directly in the genome. Combined with protein-fragment complementation assay (PCA), which enables the quantitative...

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PMID: 35524121
DOI: 10.1007/978-1-0716-2257-5_14

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