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Article

A two-step two-hybrid system to identify functionally significant protein-protein interactions.

Methods (San Diego, Calif.) - 1 Apr 2004

Poustovoitov Maxim, Serebriiskii Ilya, Adams Peter D

Abstract excerpt

The two-step two-hybrid approach described here is an adaptation of the classic two-hybrid system. Its purpose is to identify proteins that interact with a relatively small, defined, functionally significant domain of a protein of interest. In this method, a first round of screening is performed to identify proteins that interact with bait comprised of the wild type protein. Next, each of the prey identified in...

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PMID: 15003599
DOI: 10.1016/j.ymeth.2003.10.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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