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Article

Using two-hybrid interactions to identify separation-of-function mutations.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Haarer Brian, Amberg David C

Abstract excerpt

Protein functions within cells frequently require they interact physically with a number of partner proteins to coordinate the appropriate biochemical processes. Mutational analysis has been quite useful for analyzing how the loss of a gene or protein impacts cell function or more specifically particular pathways. However, the genetics approach to studying gene function can be limited by not having the right...

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PMID: 25213243
DOI: 10.1007/978-1-4939-1363-3_9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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