Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

One- plus two-hybrid system for the efficient selection of missense mutant alleles defective in protein-protein interactions.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Kim Ji Young, Park Ok Gu, Lee Young Chul

Abstract excerpt

In an effort to develop a method for the high-throughput analysis of protein interaction interfaces, we devised a novel yeast genetic screening method, termed the "one- plus two-hybrid system," which efficiently selects specific missense mutations that disrupt known protein-protein interactions. This system modifies the standard yeast two-hybrid system to allow the operation of dual reporter systems within the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genes, Reporter
Share to Genetic Vectors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutagenesis

Share to Mutation, Missense

Share to Plasmids

Share to Polymerase Chain Reaction

Share to Proteins

Share to Two-Hybrid System Techniques

Source

Identifiers

PMID: 22218862
DOI: 10.1007/978-1-61779-455-1_12

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

One- plus two-hybrid system for the efficient selection of missense mutant alleles defective in protein-protein interactions.PMID 22218862Read ↗

A two-step two-hybrid system to identify functionally significant protein-protein interactions.Semantic proximity Read ↗

A novel approach for generating full-length, high coverage allele libraries for the analysis of protein interactions.Semantic proximity Read ↗

Protein-protein interactions: the yeast two-hybrid system.Semantic proximity Read ↗

Genetic characterization of a mammalian protein-protein interaction domain by using a yeast reverse two-hybrid system.Semantic proximity Read ↗

Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions.Semantic proximity Read ↗

A bacterial/yeast merged two-hybrid system: protocol for yeast screening with single or parallel baits.Semantic proximity Read ↗

Using two-hybrid interactions to identify separation-of-function mutations.Semantic proximity Read ↗

Understanding gene and allele function with two-hybrid methods.Semantic proximity Read ↗

Analyzing protein-protein interactions using two-hybrid system.Semantic proximity Read ↗

Deep Mutational Scanning of Protein-Protein Interactions Between Partners Expressed from Their Endogenous Loci In Vivo.Semantic proximity Read ↗

The split-hybrid system. Uncoding multiprotein networks and defining mutations that affect protein interactions.Semantic proximity Read ↗

Yeast one-hybrid screens for detection of transcription factor DNA interactions.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.