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Article

A novel approach for generating full-length, high coverage allele libraries for the analysis of protein interactions.

Molecular & cellular proteomics : MCP - 1 Mar 2007

Gray Phillip N, Busser Katherine J, Chappell Thomas G

Abstract excerpt

The yeast reverse two-hybrid method was developed to identify mutations disrupting protein-protein interactions. Adoption of the method has been slow, in large part, due to the high frequency of truncation and frameshift mutants typically observed with current protocols. We have developed a new strategy, based on in vitro recombinational cloning and full-length selection in Escherichia coli, to eliminate this...

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PMID: 17151022
DOI: 10.1074/mcp.T600023-MCP200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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