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Article

A Split-Ubiquitin Based Strategy Selecting for Protein Complex-Interfering Mutations.

G3 (Bethesda, Md.) - 8 Sept 2016

Gronemeyer Thomas, Chollet Julian, Werner Stefan, Glomb Oliver, Bäuerle Anne, Johnsson Nils

Abstract excerpt

Understanding the topologies and functions of protein interaction networks requires the selective removal of single interactions. We introduce a selection strategy that enriches among a random library of alleles for mutations that impair the binding to a given partner protein. The selection makes use of a split-ubiquitin based protein interaction assay. This assay provides yeast cells that carry protein complex...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 27402358
DOI: 10.1534/g3.116.031369
PMCID: PMC5015938

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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