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Article

Site-directed mutagenesis.

Methods in enzymology - 1 Jan 2013

Bachman Julia

Abstract excerpt

Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational modifications, or to silence enzymatic activity. Alternatively,...

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PMID: 24011050
DOI: 10.1016/B978-0-12-418687-3.00019-7

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Source receipt: pubmed, retrieved 7 Sept 2026.

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