Article
Protein-protein interactions: the yeast two-hybrid system.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Guo Deyin, Rajamäki Minna-Liisa, Valkonen Jari
Abstract excerpt
Yeast two-hybrid systems are powerful tools to identify novel protein-protein interactions and have been extensively used to study viral protein interactions. The most commonly used systems are GAL4-based and LexA-based systems. Over the last decade, a range of modifications and improvements have been made to the original yeast two-hybrid system to expand the scope of molecular interaction assays and to eliminate...
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