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Article

Yeast one-hybrid screens for detection of transcription factor DNA interactions.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Ouwerkerk Pieter B F, Meijer Annemarie H

Abstract excerpt

The yeast one-hybrid system is widely recognized as a valuable and straightforward technique to study interactions between transcription factors and DNA. By means of one-hybrid screens, transcription factors or other DNA-binding proteins, expressed from cDNA expression libraries, can be identified due to the interactions with a DNA sequence-of-interest that is linked to a reporter gene, such as the yeast HIS3...

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PMID: 20931383
DOI: 10.1007/978-1-60761-682-5_16

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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