Article
Detection of a point mutation using short oligonucleotide probes in allele-specific hybridization.
BioTechniques - 1 Sept 1994
Iitiä A, Mikola M, Gregersen N, Hurskainen P, Lövgren T
Abstract excerpt
Two nonradioactive and simple procedures were developed to detect the A985G point mutation that causes medium-chain acyl-CoA deficiency. In both of these assays, short oligonucleotide probes were used in allele-specific hybridization combined with DNA amplification. The lower limit for a useful probe was found to be between 9 and 12 base pairs. Time-resolved fluorometry was utilized as the label technology and...
Topics
- Alleles
- Base Sequence
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Point Mutation