Europium-labeled oligonucleotides to detect point mutations: application to PIZ alpha 1-antitrypsin deficiency.

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Article

Europium-labeled oligonucleotides to detect point mutations: application to PIZ alpha 1-antitrypsin deficiency.

Clinical chemistry - 1 Aug 1993

Dahlén P, Carlson J, Liukkonen L, Lilja H, Siitari H, Hurskainen P, Iitä A, Jeppsson J O, Lövgren T

Abstract excerpt

We describe a novel assay for detection of point mutations. The method combines the specificity and sensitivity of the polymerase chain reaction (PCR) and allele-specific oligonucleotides (ASO) with highly sensitive time-resolved fluorometry. ASO probes differing by a single base substitution and...

Topics

Base Sequence
Biotin
Blotting, Southern
DNA
Europium
Fluorometry
Genotype
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Point Mutation

Polymerase Chain Reaction

Solutions

Time Factors

alpha 1-Antitrypsin

alpha 1-Antitrypsin Deficiency

Source

Open in PubMed

Identifiers

PMID: 8353948

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8353948-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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