Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Europium-labeled oligonucleotides to detect point mutations: application to PIZ alpha 1-antitrypsin deficiency.

Clinical chemistry - 1 Aug 1993

Dahlén P, Carlson J, Liukkonen L, Lilja H, Siitari H, Hurskainen P, Iitä A, Jeppsson J O, Lövgren T

Abstract excerpt

We describe a novel assay for detection of point mutations. The method combines the specificity and sensitivity of the polymerase chain reaction (PCR) and allele-specific oligonucleotides (ASO) with highly sensitive time-resolved fluorometry. ASO probes differing by a single base substitution and labeled with europium (Eu) chelates were hybridized in solution simultaneously with a biotinylated oligomer to a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Blotting, Southern

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genotype

Share to Molecular Sequence Data

Share to Phenotype

Share to Point Mutation

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8353948

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Europium-labeled oligonucleotides to detect point mutations: application to PIZ alpha 1-antitrypsin deficiency.PMID 8353948Read ↗

Enzyme-labelled oligonucleotides for the detection of alpha 1-antitrypsin deficiency: optimization of enzyme activity for single point mutation detection.Semantic proximity Read ↗

Competitive assay to improve the specificity of detection of single-point mutations in alpha 1-antitrypsin deficiency.Semantic proximity Read ↗

Two novel nonradioactive polymerase chain reaction-based assays of dried blood spots, genomic DNA, or whole cells for fast, reliable detection of Z and S mutations in the alpha 1-antitrypsin gene.Semantic proximity Read ↗

Detection of alpha 1-antitrypsin Z and S mutations by polymerase chain reaction-mediated site-directed mutagenesis.Semantic proximity Read ↗

Simple triple-label detection of seven cystic fibrosis mutations by time-resolved fluorometry.Semantic proximity Read ↗

Detection of a point mutation using short oligonucleotide probes in allele-specific hybridization.Semantic proximity Read ↗

Detection of genetic variants of alpha 1-antitrypsin with site-specific monoclonal antibodies.Semantic proximity Read ↗

Rapid PCR real-time genotyping of M-Malton alpha1-antitrypsin deficiency alleles by molecular beacons.Semantic proximity Read ↗

Denaturing gradient gel electrophoresis of the alpha 1-antitrypsin gene: application to prenatal diagnosis.Semantic proximity Read ↗

Polymerase chain reaction-mediated site-directed mutagenesis detection of Z and S alpha-1-antitrypsin alleles in family members.Semantic proximity Read ↗

Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.Semantic proximity Read ↗

Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.