Article
An alternate nonisotopic technique of PCR-mediated allele-specific oligonucleotide analysis for the detection of a point mutation.
Research communications in chemical pathology and pharmacology - 1 Jan 1993
Nakajima H, Hamaguchi T, Tomita K, Uenaka R, Yamagata K, Hanafusa T, Kuwajima M, Noguchi T, Tanaka T, Kono N
Abstract excerpt
We report a simple protocol for the allele analysis of a point mutation using polymerase chain reaction. Conditions have been determined to use probes prepared for the conventional allele-specific oligonucleotide hybridization method directly. After amplification of the genomic region containing...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Probes
- Gene Amplification
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotides
- Point Mutation
- Polymerase Chain Reaction