Allele-specific hybridization of lipoprotein lipase and factor-V Leiden missense mutations with direct label alkaline phosphatase-conjugated oligonucleotide probes.

doi:10.1016/1050-3862(95)00149-2

Article

Allele-specific hybridization of lipoprotein lipase and factor-V Leiden missense mutations with direct label alkaline phosphatase-conjugated oligonucleotide probes.

Genetic analysis : biomolecular engineering - 1 Sept 1996

Vary C P, Carmody M, LeBlanc R, Hayes T, Rundell C, Keilson L

Abstract excerpt

Direct label alkaline phosphatase (AP) conjugated oligonucleotide probes (AP-DNA) were prepared to assess their utility for allele-specific detection of single base substitutions. Oligonucleotide conjugates were designed to detect point mutations in the genes for lipoprotein lipase (LPL) and coag...

Topics

Alkaline Phosphatase
Alleles
Blotting, Southern
Factor V
Humans
Lipoprotein Lipase
Oligonucleotide Probes
Point Mutation
Polymerase Chain Reaction

Allele-specific hybridization of lipoprotein lipase and factor-V Leiden missense mutations with direct label alkaline phosphatase-conjugated oligonucleotide probes.

Abstract excerpt

Topics

Discuss the evidence