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Article

Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

Molecular and cellular probes - 1 Dec 1992

Iitiä A, Liukkonen L, Siitari H

Abstract excerpt

A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood disks, normally used in neonatal screening programmes, by boiling in alkaline solution. A 138 bp...

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PMID: 1480190
DOI: 10.1016/0890-8508(92)90047-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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