Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

doi:10.1016/0890-8508(92)90047-2

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

Molecular and cellular probes - 1 Dec 1992

Iitiä A, Liukkonen L, Siitari H

Abstract excerpt

A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood disks, normally used in neonatal screening programmes, by boiling in alkaline solution. A 138 bp...

Topics

Alleles
Base Sequence
Blood Proteins
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
DNA
DNA Probes
Europium
Fluorometry
Genes
Genetic Carrier Screening

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Infant, Newborn

Molecular Sequence Data

Nucleic Acid Hybridization

Polymerase Chain Reaction

Samarium

Sequence Deletion

Source

Open in PubMed

Identifiers

PMID: 1480190
DOI: 10.1016/0890-8508(92)90047-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1480190-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.PMID 1480190Read ↗

Simple triple-label detection of seven cystic fibrosis mutations by time-resolved fluorometry.Semantic proximity Read ↗

Detection of mutation delta F508 in the cystic fibrosis gene using allele-specific PCR primers and time-resolved fluorometry.Semantic proximity Read ↗

Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing.Semantic proximity Read ↗

A fast and robust dual-label nonradioactive oligonucleotide ligation assay for detection of factor V Leiden.Semantic proximity Read ↗

Detection of HLA-B27 alleles by group-specific amplification and time-resolved fluorometry.Semantic proximity Read ↗

Biosensor technology for real-time detection of the cystic fibrosis W1282X mutation in CFTR.Semantic proximity Read ↗

Allele specific PCR with microfluorometry: application to the detection of del F508 mutation in cystic fibrosis.Semantic proximity Read ↗

Methylene blue as an electrochemical indicator for DF508 cystic fibrosis mutation detection.Semantic proximity Read ↗

DNA enzyme immunoassay: a rapid and convenient colorimetric method for diagnosis of cystic fibrosis.Semantic proximity Read ↗

Detection of a point mutation using short oligonucleotide probes in allele-specific hybridization.Semantic proximity Read ↗

Dual-color detection of DNA sequence variants by ligase-mediated analysis.Semantic proximity Read ↗

Cystic Fibrosis Mutation Detection with SPR Biosensor in Real Samples via Multiple Surfaces Binding Method.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.