Article
A fluorogenic allele-specific amplification method for DNA-based screening for inherited metabolic disorders.
Acta paediatrica (Oslo, Norway : 1992). Supplement - 1 Dec 1999
Matsubara Y, Fujii K, Rinaldo P, Narisawa K
Abstract excerpt
Rapidly growing mutation databases for various inherited metabolic diseases raise the possibility of neonatal screening by DNA-based diagnosis. It is therefore important to develop a simple DNA diagnostic method which is suitable for processing a large number of samples. We have devised an allele-specific amplification (ASA) method with a fluorogenic probe (TaqMan probe) to detect point mutations. A pairwise PCR...
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