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Article

Simple triple-label detection of seven cystic fibrosis mutations by time-resolved fluorometry.

Clinical chemistry - 1 Jul 1997

Heinonen P, Iitiä A, Torresani T, Lövgren T

Abstract excerpt

We describe a simple hybridization assay performed in microtitration wells with use of DNA probes labeled with three different lanthanide chelates for detection of seven mutations that cause cystic fibrosis. The assay is based on DNA amplification of four fragments containing the mutations (delta F508, G1717-->A, G542X, R553X, 3905 insertion T, W1282X, and N1303K) by PCR, followed by hybridization with short,...

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PMID: 9216449

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