Application of synthetic oligonucleotides to the diagnosis of human genetic diseases.

doi:10.1016/s0300-9084(85)80164-4

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Article

Application of synthetic oligonucleotides to the diagnosis of human genetic diseases.

Biochimie - 1 Jan 2000

Wallace R B, Studencki A B, Murasugi A

Abstract excerpt

Under appropriate conditions synthetic oligonucleotide hybridization probes display essentially absolute hybridization specificity. That is, every nucleotide must form a Watson-Crick base pair in order that the probe forms a stable duplex. All of the non-Watson-Crick base pairs, including G-T, ha...

Topics

Alleles
Amino Acid Sequence
Base Composition
Base Sequence
Genetic Diseases, Inborn
Genetic Engineering
Genotype
Humans
Mutation
Nucleic Acid Hybridization
Oligodeoxyribonucleotides
Plasmids

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