Article
Detection of human DNA mutations with nonradioactive, allele-specific oligonucleotide probes.
Pharmacogenetics - 1 Apr 1992
Hajra A, Sorenson R C, La Du B N
Abstract excerpt
We describe a method of detecting human DNA mutations with nonradioactive, biotinylated allele-specific oligonucleotide probes. This method can detect seven different mutations in the butyrylcholinesterase, cystic fibrosis, and N-acetyltransferase genes under identical assay conditions. This indicates that it may be used to detect mutations responsible for a wide variety of genetic diseases and pharmacogenetic...
Topics
- Alleles
- Arylamine N-Acetyltransferase
- Base Sequence
- Biotin
- Butyrylcholinesterase
- Cystic Fibrosis
- DNA
- DNA Mutational Analysis
- Humans
- Molecular Probe Techniques
- Molecular Sequence Data