Article
A single amino acid substitution in the H-2Kb molecule generates a defined allogeneic epitope.
Molecular immunology - 1 Dec 1993
Kesari K V, Van Bleek G, Nathenson S G, Geliebter J
Abstract excerpt
Using Mitomycin C mutagenesis and negative and positive selection with monoclonal antibodies specific for H-2Kb and H-2Kbm10, respectively, a mutant cell line clone, Mitc-182, was isolated. Direct sequencing of uncloned cDNA as well as PCR based cloning and sequencing of the H-2Kb182 transcript from this mutant revealed a single G-->T transversion resulting in the substitution of Trp167 by cysteine....
Topics
- Animals
- Antibodies, Monoclonal
- Antigen Presentation
- Antigens, Viral
- Base Sequence
- Epitopes
- H-2 Antigens
- Isoantibodies
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Molecular Sequence Data