Article
Induction of microvariant-specific CTL lines reactive to a single amino acid mismatch in bulk cultures using a transfectant expressing a single HLA class I molecule.
Journal of immunology (Baltimore, Md. : 1950) - 1 Jan 1996
Cereb N, Yang S Y
Abstract excerpt
Alloreactivity against micromismatches in MHC class I molecules is difficult to measure. Here, we describe an in vitro model with which it is possible to examine alloreactivity against a single HLA class I allotype. The HLA class I- and class II-negative myelocytic leukemia cell line K562 was tra...
Topics
- Alleles
- Epitopes
- Genes, MHC Class I
- Genetic Variation
- HLA-B Antigens
- Humans
- Isoantigens
- Leukemia, Myeloid
- Lymphocyte Culture Test, Mixed
- Multigene Family
- T-Lymphocytes
- T-Lymphocytes, Cytotoxic
- Transfection
- Tumor Cells, Cultured