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Article

Abnormally high incidence of SCE in three successive cell cycles in the CHO mutant EM9 as detected by a three-way immunoperoxidase differential staining.

Mutation research - 1 Dec 1993

Piñero J, Cortés F

Abstract excerpt

A high resolution three-way immunoperoxidase method for the detection of SCE per cell generation in chromosomes with very low levels of BrdU in DNA has been recently standardized in our laboratory. We have made use of this methodology to assess whether the CHO mutant EM9 is solely hypersensitive to BrdU incorporated into template DNA as regards the extraordinarily high SCE frequency observed as compared with its...

Topics

Animals
Bromodeoxyuridine
CHO Cells
Cell Cycle
Cricetinae
DNA
DNA Replication
Immunoenzyme Techniques
Mutation
Sister Chromatid Exchange
Staining and Labeling

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 7504180
DOI: 10.1016/0165-1161(93)90023-s

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7504180-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Abnormally high incidence of SCE in three successive cell cycles in the CHO mutant EM9 as detected by a three-way immunoperoxidase differential staining.PMID 7504180Read ↗

A high yield of translocations parallels the high yield of sister chromatid exchanges in the CHO mutant EM9.Semantic proximity Read ↗

High yield of endoreduplication induced by ICRF-193: a topoisomerase II catalytic inhibitor.Semantic proximity Read ↗

Cytogenetic effects of inhibition of topoisomerase I or II activities in the CHO mutant EM9 and its parental line AA8.Semantic proximity Read ↗

DNA-strand breaks associated with halogenated pyrimidine incorporation.Semantic proximity Read ↗

The high rate of endoreduplication in the repair deficient CHO mutant EM9 parallels a reduced level of methylated deoxycytidine in DNA.Semantic proximity Read ↗

Higher G2 sensitivity to the induction of chromosomal damage in the CHO mutant EM9 than in its parental line AA8 by camptothecin, an inhibitor of DNA topoisomerase I.Semantic proximity Read ↗

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