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Article

Engineering of T7 DNA-dependent RNA polymerase with activity at elevated temperature.

PloS one - 1 Jan 2026

Hehn Svenja, Gutbrod Julia, Gutjahr Maxi, Marx Andreas

Abstract excerpt

Bacteriophage T7 RNA polymerase (T7 RNAP) is a key enzyme for in vitro transcription (IVT) and plays a central role in the production of synthetic mRNA for research and therapeutic applications. However, IVT frequently generates double-stranded RNA (dsRNA) as an undesired by-product, which can trigger innate immune responses and compromise mRNA quality. Increasing reaction temperatures reduces dsRNA formation,...

Topics

DNA-Directed RNA Polymerases
Viral Proteins
Bacteriophage T7
Transcription, Genetic
Protein Engineering
Models, Molecular
Temperature
Mutation
RNA, Double-Stranded
Hot Temperature

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Identifiers

PMID: 42475319
DOI: 10.1371/journal.pone.0353775
PMCID: PMC13384301

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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