Article
A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymerase.
RNA biology - 15 Oct 2021
Wu Hui, Wei Ting, Yu Bingbing, Cheng Rui, Huang Fengtao, Lu Xuelin, Yan Yan, Wang Xionglue, Liu Chenli, Zhu Bin
Abstract excerpt
Transcription termination is one of the least understood processes of gene expression. As the prototype model for transcription studies, the single-subunit T7 RNA polymerase (RNAP) is known to respond to two types of termination signals, but the mechanism underlying such termination, especially the specific elements of the polymerase involved, is still unclear, due to a lack of knowledge with respect to the...
Topics
- Bacteriophage T7
- DNA-Directed RNA Polymerases
- Escherichia coli
- Mutation
- Promoter Regions, Genetic
- RNA-Dependent RNA Polymerase
- Transcription Termination, Genetic
- Transcription, Genetic
- Viral Proteins