Article
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase.
Science (New York, N.Y.) - 15 Nov 2002
Yin Y Whitney, Steitz Thomas A
Abstract excerpt
To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2.1 angstrom resolution the crystal structure of a T7 RNAP elongation complex with 30 base pairs of duplex DNA containing a "transcription bubble" interacting with a 17-nucleotide RNA transcript. The...
Topics
- Bacteriophage T7
- Binding Sites
- Crystallization
- Crystallography, X-Ray
- DNA
- DNA-Directed RNA Polymerases
- Models, Molecular
- Mutation
- N-Acetylmuramoyl-L-alanine Amidase
- Nucleic Acid Heteroduplexes
- Promoter Regions, Genetic