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Article

A mutant T7 RNA polymerase as a DNA polymerase.

The EMBO journal - 15 Sept 1995

Sousa R, Padilla R

Abstract excerpt

We have identified a T7 RNA polymerase (RNAP) mutant that efficiently utilizes deoxyribonucleoside triphosphates. In vitro this mutant will synthesize RNA, DNA or 'transcripts' of mixed dNMP/rNMP composition depending on the mix of NTPs present in the synthesis reaction. The mutation is conservative, changes Tyr639 within the active site to phenylalanine and does not affect promoter specificity or overall...

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Identifiers

PMID: 7556104
DOI: 10.1002/j.1460-2075.1995.tb00140.x
PMCID: PMC394553

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Source receipt: pubmed, retrieved 7 Sept 2026.

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A mutant T7 RNA polymerase as a DNA polymerase.PMID 7556104Read ↗

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