Article
A mutant T7 RNA polymerase as a DNA polymerase.
The EMBO journal - 15 Sept 1995
Sousa R, Padilla R
Abstract excerpt
We have identified a T7 RNA polymerase (RNAP) mutant that efficiently utilizes deoxyribonucleoside triphosphates. In vitro this mutant will synthesize RNA, DNA or 'transcripts' of mixed dNMP/rNMP composition depending on the mix of NTPs present in the synthesis reaction. The mutation is conservat...
Topics
- Bacteriophage T7
- Base Sequence
- DNA
- DNA Primers
- DNA-Directed DNA Polymerase
- DNA-Directed RNA Polymerases
- Deoxyribonucleotides
- Genetic Code
- Kinetics
- Molecular Sequence Data
- Mutation
- Polynucleotides
- RNA
- Ribonucleotides
- Substrate Specificity
- Transcription, Genetic
- Viral Proteins