Article

A sequence-to-function model to predict T7 transcription rates and redesign T7 expression systems with lowered production of immunogenic RNA byproducts

2026-08-03

Abstract excerpt

T7 RNA polymerase is widely used to produce RNA using a canonical T7 promoter; however, it will also bind to low-affinity sites to generate cryptic transcription and produce RNA byproducts, which reduce full-length mRNA purity and yield. When manufacturing therapeutic RNAs for clinical applications, RNA byproducts must be removed using costly downstream purification and can cause adverse immunogenicity. To predict...

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Literature Corpus work: 11700e7a-3af8-5d9b-bf45-2cf6d8c03245
DOI: 10.64898/2026.08.01.742228

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