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Article

DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants.

Nucleic acids research - 12 Aug 2024

Yu Bingbing, Chen Yifan, Yan Yan, Lu Xueling, Zhu Bin

Abstract excerpt

The remarkable success of messenger RNA (mRNA)-based vaccines has underscored their potential as a novel biotechnology platform for vaccine development and therapeutic protein delivery. However, the single-subunit RNA polymerase from bacteriophage T7 widely used for in vitro transcription is well known to generate double-stranded RNA (dsRNA) by-products that strongly stimulate the mammalian innate immune...

Topics

DNA-Directed RNA Polymerases
Viral Proteins
Transcription, Genetic
Mutation
RNA, Double-Stranded
Animals
DNA
RNA, Messenger
Bacteriophage T7
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 38979568
DOI: 10.1093/nar/gkae593
PMCID: PMC11317132

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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