Article
Directed evolution of DNA polymerase, RNA polymerase and reverse transcriptase activity in a single polypeptide.
Journal of molecular biology - 18 Aug 2006
Ong Jennifer L, Loakes David, Jaroslawski Szymon, Too Kathleen, Holliger Philipp
Abstract excerpt
DNA polymerases enable key technologies in modern biology but for many applications, native polymerases are limited by their stringent substrate recognition. Here we describe short-patch compartmentalized self-replication (spCSR), a novel strategy to expand the substrate spectrum of polymerases in a targeted way. spCSR is based on the previously described CSR, but unlike CSR only a short region (a "patch") of the...
Topics
- Amino Acid Sequence
- DNA
- DNA Polymerase I
- DNA-Directed RNA Polymerases
- Deoxyribonucleotides
- Directed Molecular Evolution
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- RNA