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Article

Minimizing dsRNA impurity generation through structure-function-guided directed evolution of T7 RNA polymerase.

International journal of biological macromolecules - 1 May 2026

He Wei, Xi Jingchao, Wu Xiaoqin, Geng Qi, Zhang Xinya, Ye Jianren, He Yu-Cai, Jin Qiuheng

Abstract excerpt

The presence of dsRNA, a prominent pathogen-associated molecular pattern, poses a significant challenge for mRNA therapeutics due to its potent activation of innate immune responses. Extensive research has been conducted in the field in recent years, leading to the discovery of various strategies for dsRNA reduction. Among these approaches, T7 RNA polymerase (T7 RNAP) engineering stands out as the most...

Topics

DNA-Directed RNA Polymerases
Viral Proteins
RNA, Double-Stranded
Directed Molecular Evolution
Mutation
Transcription, Genetic
Structure-Activity Relationship

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 41932467
DOI: 10.1016/j.ijbiomac.2026.151789

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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