Article
Transcription yield of fully 2'-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants.
Nucleic acids research - 3 Sept 2015
Meyer Adam J, Garry Daniel J, Hall Bradley, Byrom Michelle M, McDonald Hannah G, Yang Xu, Yin Y Whitney, Ellington Andrew D
Abstract excerpt
On average, mutations are deleterious to proteins. Mutations conferring new function to a protein often come at the expense of protein folding or stability, reducing overall activity. Over the years, a panel of T7 RNA polymerases have been designed or evolved to accept nucleotides with modified ribose moieties. These modified RNAs have proven useful, especially in vivo, but the transcriptional yields tend to be...
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